Plasmid

Part:BBa_I714038:Design

Designed by: Anting Xu   Group: iGEM07_Peking   (2007-10-26)


pSC101 with relaxase and oriT deleted


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 4027
    Illegal PstI site found at 2936
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
    Illegal NheI site found at 7630
    Illegal SpeI site found at 4027
    Illegal PstI site found at 2936
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 375
    Illegal XhoI site found at 7013
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 4027
    Illegal PstI site found at 2936
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 4027
    Illegal PstI site found at 2936
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1283
    Illegal NgoMIV site found at 5479
    Illegal NgoMIV site found at 7568
    Illegal AgeI site found at 1687
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Region of deletion: 2936bp - 4115bp (containing mobA and oriT) and substituted with PstI restriction site CTGCAG

Method of deletion: in vitro reversed cyclic PCR and self-ligation.


Source

plasmid pSC101. Genebank NC_002056.

References